Abstract
The need for alternate and sustainable treatment methods has increased due to the rising incidence of microbial diseases and the quick development of antimicrobial resistance (AMR). A wealth of bioactive phytochemicals with antibacterial, anti-inflammatory, antioxidant, and wound-healing qualities can be found in medicinal plants. The benefits of spray-based topical formulations over traditional dose forms—such as ease of application, consistent distribution, better sanitation, and increased patient compliance—have drawn a lot of attention in recent years. The function of botanically derived phytochemicals in antimicrobial spray formulations is critically examined in this review, with a focus on the compounds' botanical origins, chemical makeup, antimicrobial action mechanisms, and formulation significance. There is a thorough discussion of well-known medicinal herbs such Syzygium aromaticum, Terminalia chebula, Ocimum sanctum, Aloe vera, Curcuma longa, and Azadirachta indica. Moreover, formulation strategies, benefits of plant-derived antimicrobial sprays, existing research deficiencies, and future possibilities are emphasized, highlighting the significance of combining traditional botanical knowledge with contemporary phytopharmaceutical technologies.
Keywords
References
- Alfuraydi, A. A., Aziz, I. M., & Almajhdi, F. N. (2024). Assessment of antioxidant, anticancer, and antibacterial activities of the rhizome of ginger (Zingiber officinale). Journal of King Saud University - Science, 36(3), 103112. https://doi.org/10.1016/j.jksus.2024.103112 [Google Scholar]
- Azad, M. A. K., Barwal, S. K., & Moni, A. (2023). Exploring the impact of integrated breeding strategies in enhancing yield, nutritional quality, and stress tolerance in alfalfa. Plant Trends, 1(1), 1-15. [Google Scholar]
- Bag, A., Bhattacharyya, S. K., & Chattopadhyay, R. R. (2013). The development of Terminalia chebula Retz. (Combretaceae) in clinical research. Asian Pacific Journal of Tropical Biomedicine, 3(3), 244–252. https://doi.org/10.1016/s2221-1691(13)60059-3 [Google Scholar]
- Chauhan, C., Goutam, C., Kumar, S., Singh, I., & Yerramilli, V. (2022). GC-MS analysis and phytochemical screening of chloroform extract of Amaranthus viridis. The Journal of the Indian Botanical Society, 102(01), 53-60. [Google Scholar]
- Cortés-Rojas, D. F., De Souza, C. R. F., & Oliveira, W. P. (2014). Clove (Syzygium aromaticum): a precious spice. Asian Pacific Journal of Tropical Biomedicine, 4(2), 90-96. [Google Scholar]
- Finina, B. F., & Mersha, A. K. (2024). Nano-enabled antimicrobial thin films: design and mechanism of action. RSC Advances, 14(8), 5290-5308. [Google Scholar]
- Hemmingsen, L., Sørensen, M., & Hansen, J. (2021). Chitosan-based antimicrobial materials and their biomedical applications. Carbohydrate Polymers, 255, 117–143. [Google Scholar]
- Iweala, E. J., Uche, M. E., Dike, E. D., Etumnu, L. R., Dokunmu, T. M., Oluwapelumi, A. E., & Ugbogu, E. A. (2023). Curcuma longa (Turmeric): Ethnomedicinal uses, phytochemistry, pharmacological activities and toxicity profiles—A review. Pharmacological Research-Modern Chinese Medicine, 6, 100222. [Google Scholar]
- Kalelkar, P. P., Riddick, M., & García, A. J. (2022). Biomaterial-based antimicrobial therapies for wound healing. Biomaterials Science, 10(5), 1221–1242. [Google Scholar]
- Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PloS one, 16(3), e0249253. [Google Scholar]
- Khameneh, B., Eskin, N. M., Iranshahy, M., & Fazly Bazzaz, B. S. (2021). Phytochemicals: a promising weapon in the arsenal against antibiotic-resistant bacteria. Antibiotics, 10(9), 1044. [Google Scholar]
- Kumar, A., Das, S., Ali, S., Jaiswal, S. G., Rabbani, A., Rahman, S. M. E., & Wei, S. (2025). Mechanisms, applications and challenges of natural antimicrobials in food system. Food Bioscience, 107864. [Google Scholar]
- Mera, R., Torres, A., & Ríos, J. L. (2019). Secondary metabolites from plants as potential antimicrobial agents: A review. Phytochemistry Reviews, 18, 1199–1215. [Google Scholar]
- Mihociu, T. E., Cimponeriu, D. G., Mustatea, G., &Belc, N. (2024). Bioaccessibility and stability of phytochemical compounds, essential features in the design of functional foods: a review. Journal of Hygienic Engineering & Design, 46(1), 1-10. [Google Scholar]
- Pacheco, R. L., Latorraca, C. D. O. C., Pires dos Santos, A. P., Martimbianco, A. L. C., Moreira, R. D. F. C., Logullo, P., & Riera, R. (2022). Efficacy and safety of home-based intravenous antibiotic therapy among adults: a systematic review. International Journal of Antimicrobial Agents, 59(4), 106555. https://doi.org/10.1016/j.ijantimicag.2022.106555 [Google Scholar]
- Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41 [Google Scholar]
- Pattanayak, P., Behera, P., Das, D., & Panda, S. K. (2010). Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy Reviews, 4(7), 95. [Google Scholar]
- Pinto, L., Tapia-Rodríguez, M. R., Baruzzi, F., & Ayala-Zavala, J. F. (2023). Plant antimicrobials for food quality and safety: Recent views and future challenges. Foods, 12(12), 2315. [Google Scholar]
- Safarzadeh, S., Shirban, F., Bagherniya, M., Sathyapalan, T., & Sahebkar, A. (2021). The effects of herbal medicines on cancer therapy‐induced oral mucositis: A literature review. Phytotherapy Research, 36(1), 243–265. https://doi.org/10.1002/ptr.7308 [Google Scholar]
- Salam, M. A., Al-Amin, M. Y., Salam, M. T., Pawar, J., Akhter, N., Rabaan, A. A., & Alqumber, M. A. A. (2023). Antimicrobial Resistance: A Growing Serious Threat for Global Public Health. Preprint. https://doi.org/10.20944/preprints202305.0555.v1 [Google Scholar]
- Shah, S. H., Kumar, S., & Yerramilli, V. (2021). An insight on the ascorbate peroxidase and glutathione reductase activities in plants under salinity stress: mini review. Research Journal of Agricultural Science, 12, 1558-1563. [Google Scholar]
- Singh, T. B., Kumar, S., Singh, D. K., Khirwar, S. S., & Agnihotri, R. K. (2019). Toxicity of Cr and Pb during vegetative growth of Sesamum indicum L. The Journal of Indian Botanical Society, 98(3and4), 219-226. [Google Scholar]
- Sharma, A., & Joshi, R. (2016). Antimicrobial activity of medicinal plants. International Journal of Pharmaceutical Sciences, 8(3), 12–18. [Google Scholar]
- Sayed, G. A., Hal, D. M., Abd El Hafeez, M. S., Shatat, A. A. S., Salman, A., Eisa, N. M., Ramadan, A., El-Shiekh, R. A., Hatem, S., & Aly, S. H. (2023). Herbal remedies for oral and dental health: A comprehensive review of their antimicrobial, anti-inflammatory, and antioxidant mechanisms. Inflammopharmacology, https://doi.org/10.1007/s10787-024-01631-8 [Google Scholar]
- Sánchez Hernández, E. (2024). Novel antimicrobial formulations based on plant extracts for agroforestry applications. Universidad de Valladolid. Escuela de Doctorado. https://doi.org/10.35376/10324/66919 [Google Scholar]
- Shrestha, L., Fan, H. M., Tao, H. R., & Huang, J. D. (2022). Recent strategies to combat biofilms using antimicrobial agents and therapeutic approaches. Pathogens, 11(3), 292. [Google Scholar]
- Surjushe, A., Vasani, R., & Saple, D. (2008). Aloe vera: a short review. Indian Journal of Dermatology, 53(4), 163-166. [Google Scholar]
- Teles, A. M., Rosa, T. D. D. S., Mouchrek, A. N., Abreu-Silva, A. L., Calabrese, K. D. S., & Almeida-Souza, F. (2019). Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity. Evidence-Based Complementary and Alternative Medicine, 2019, 1–12. https://doi.org/10.1155/2019/2421695 [Google Scholar]
- Wang, C., Su, Y., Shahriar, S. S., Li, Y., & Xie, J. (2024). Emerging strategies for treating medical device and wound‐associated biofilm infections. Microbial Biotechnology, 17(10), e70035. [Google Scholar]
- Wylie, M. R., & Merrell, D. S. (2022). The antimicrobial potential of the neem tree Azadirachta indica. Frontiers in Pharmacology, 13, 891535. [Google Scholar]
- Yang, X., Wang, Y., Li, J., Zhang, L., & Zhao, Y. (2023). Plant-derived natural products as potential antimicrobial agents against drug-resistant pathogens: A review. Frontiers in Microbiology, 14, 1187853. https://doi.org/10.3389/fmicb.2023.1187853 [Google Scholar]
- Zhou, K., Shi, M., Chen, R., Zhang, Y., Sheng, Y., Tong, C., & Shou, D. (2025). Natural phytochemical-based strategies for antibiofilm applications. Chinese Medicine, 20(1), 96. [Google Scholar]

