Botanically Derived Phytochemicals in Antimicrobial Spray Formulations: Mechanism, Strategies and Future Prospects

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AgroEnvironmental Sustainability
Vansh Gupta , Gayatri Saini , Anshul , Archit Kapil , Mahvish Khan , Sandeep Kumar Barwal , Harsh Singh

Abstract

The need for alternate and sustainable treatment methods has increased due to the rising incidence of microbial diseases and the quick development of antimicrobial resistance (AMR). A wealth of bioactive phytochemicals with antibacterial, anti-inflammatory, antioxidant, and wound-healing qualities can be found in medicinal plants. The benefits of spray-based topical formulations over traditional dose forms—such as ease of application, consistent distribution, better sanitation, and increased patient compliance—have drawn a lot of attention in recent years. The function of botanically derived phytochemicals in antimicrobial spray formulations is critically examined in this review, with a focus on the compounds' botanical origins, chemical makeup, antimicrobial action mechanisms, and formulation significance. There is a thorough discussion of well-known medicinal herbs such Syzygium aromaticum, Terminalia chebula, Ocimum sanctum, Aloe vera, Curcuma longa, and Azadirachta indica. Moreover, formulation strategies, benefits of plant-derived antimicrobial sprays, existing research deficiencies, and future possibilities are emphasized, highlighting the significance of combining traditional botanical knowledge with contemporary phytopharmaceutical technologies.

Keywords

antimicrobial Azadirachta indica Ocimum sanctum secondary metabolties

References

  1. Alfuraydi, A. A., Aziz, I. M., & Almajhdi, F. N. (2024). Assessment of antioxidant, anticancer, and antibacterial activities of the rhizome of ginger (Zingiber officinale). Journal of King Saud University - Science, 36(3), 103112. https://doi.org/10.1016/j.jksus.2024.103112 [Google Scholar]
  2. Azad, M. A. K., Barwal, S. K., & Moni, A. (2023). Exploring the impact of integrated breeding strategies in enhancing yield, nutritional quality, and stress tolerance in alfalfa. Plant Trends, 1(1), 1-15. [Google Scholar]
  3. Bag, A., Bhattacharyya, S. K., & Chattopadhyay, R. R. (2013). The development of Terminalia chebula Retz. (Combretaceae) in clinical research. Asian Pacific Journal of Tropical Biomedicine, 3(3), 244–252. https://doi.org/10.1016/s2221-1691(13)60059-3 [Google Scholar]
  4. Chauhan, C., Goutam, C., Kumar, S., Singh, I., & Yerramilli, V. (2022). GC-MS analysis and phytochemical screening of chloroform extract of Amaranthus viridis. The Journal of the Indian Botanical Society, 102(01), 53-60. [Google Scholar]
  5. Cortés-Rojas, D. F., De Souza, C. R. F., & Oliveira, W. P. (2014). Clove (Syzygium aromaticum): a precious spice. Asian Pacific Journal of Tropical Biomedicine, 4(2), 90-96. [Google Scholar]
  6. Finina, B. F., & Mersha, A. K. (2024). Nano-enabled antimicrobial thin films: design and mechanism of action. RSC Advances, 14(8), 5290-5308. [Google Scholar]
  7. Hemmingsen, L., Sørensen, M., & Hansen, J. (2021). Chitosan-based antimicrobial materials and their biomedical applications. Carbohydrate Polymers, 255, 117–143. [Google Scholar]
  8. Iweala, E. J., Uche, M. E., Dike, E. D., Etumnu, L. R., Dokunmu, T. M., Oluwapelumi, A. E., & Ugbogu, E. A. (2023). Curcuma longa (Turmeric): Ethnomedicinal uses, phytochemistry, pharmacological activities and toxicity profiles—A review. Pharmacological Research-Modern Chinese Medicine, 6, 100222. [Google Scholar]
  9. Kalelkar, P. P., Riddick, M., & García, A. J. (2022). Biomaterial-based antimicrobial therapies for wound healing. Biomaterials Science, 10(5), 1221–1242. [Google Scholar]
  10. Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PloS one, 16(3), e0249253. [Google Scholar]
  11. Khameneh, B., Eskin, N. M., Iranshahy, M., & Fazly Bazzaz, B. S. (2021). Phytochemicals: a promising weapon in the arsenal against antibiotic-resistant bacteria. Antibiotics, 10(9), 1044. [Google Scholar]
  12. Kumar, A., Das, S., Ali, S., Jaiswal, S. G., Rabbani, A., Rahman, S. M. E., & Wei, S. (2025). Mechanisms, applications and challenges of natural antimicrobials in food system. Food Bioscience, 107864. [Google Scholar]
  13. Mera, R., Torres, A., & Ríos, J. L. (2019). Secondary metabolites from plants as potential antimicrobial agents: A review. Phytochemistry Reviews, 18, 1199–1215. [Google Scholar]
  14. Mihociu, T. E., Cimponeriu, D. G., Mustatea, G., &Belc, N. (2024). Bioaccessibility and stability of phytochemical compounds, essential features in the design of functional foods: a review. Journal of Hygienic Engineering & Design, 46(1), 1-10. [Google Scholar]
  15. Pacheco, R. L., Latorraca, C. D. O. C., Pires dos Santos, A. P., Martimbianco, A. L. C., Moreira, R. D. F. C., Logullo, P., & Riera, R. (2022). Efficacy and safety of home-based intravenous antibiotic therapy among adults: a systematic review. International Journal of Antimicrobial Agents, 59(4), 106555. https://doi.org/10.1016/j.ijantimicag.2022.106555 [Google Scholar]
  16. Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41 [Google Scholar]
  17. Pattanayak, P., Behera, P., Das, D., & Panda, S. K. (2010). Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy Reviews, 4(7), 95. [Google Scholar]
  18. Pinto, L., Tapia-Rodríguez, M. R., Baruzzi, F., & Ayala-Zavala, J. F. (2023). Plant antimicrobials for food quality and safety: Recent views and future challenges. Foods, 12(12), 2315. [Google Scholar]
  19. Safarzadeh, S., Shirban, F., Bagherniya, M., Sathyapalan, T., & Sahebkar, A. (2021). The effects of herbal medicines on cancer therapy‐induced oral mucositis: A literature review. Phytotherapy Research, 36(1), 243–265. https://doi.org/10.1002/ptr.7308 [Google Scholar]
  20. Salam, M. A., Al-Amin, M. Y., Salam, M. T., Pawar, J., Akhter, N., Rabaan, A. A., & Alqumber, M. A. A. (2023). Antimicrobial Resistance: A Growing Serious Threat for Global Public Health. Preprint. https://doi.org/10.20944/preprints202305.0555.v1 [Google Scholar]
  21. Shah, S. H., Kumar, S., & Yerramilli, V. (2021). An insight on the ascorbate peroxidase and glutathione reductase activities in plants under salinity stress: mini review. Research Journal of Agricultural Science, 12, 1558-1563. [Google Scholar]
  22. Singh, T. B., Kumar, S., Singh, D. K., Khirwar, S. S., & Agnihotri, R. K. (2019). Toxicity of Cr and Pb during vegetative growth of Sesamum indicum L. The Journal of Indian Botanical Society, 98(3and4), 219-226. [Google Scholar]
  23. Sharma, A., & Joshi, R. (2016). Antimicrobial activity of medicinal plants. International Journal of Pharmaceutical Sciences, 8(3), 12–18. [Google Scholar]
  24. Sayed, G. A., Hal, D. M., Abd El Hafeez, M. S., Shatat, A. A. S., Salman, A., Eisa, N. M., Ramadan, A., El-Shiekh, R. A., Hatem, S., & Aly, S. H. (2023). Herbal remedies for oral and dental health: A comprehensive review of their antimicrobial, anti-inflammatory, and antioxidant mechanisms. Inflammopharmacology, https://doi.org/10.1007/s10787-024-01631-8 [Google Scholar]
  25. Sánchez Hernández, E. (2024). Novel antimicrobial formulations based on plant extracts for agroforestry applications. Universidad de Valladolid. Escuela de Doctorado. https://doi.org/10.35376/10324/66919 [Google Scholar]
  26. Shrestha, L., Fan, H. M., Tao, H. R., & Huang, J. D. (2022). Recent strategies to combat biofilms using antimicrobial agents and therapeutic approaches. Pathogens, 11(3), 292. [Google Scholar]
  27. Surjushe, A., Vasani, R., & Saple, D. (2008). Aloe vera: a short review. Indian Journal of Dermatology, 53(4), 163-166. [Google Scholar]
  28. Teles, A. M., Rosa, T. D. D. S., Mouchrek, A. N., Abreu-Silva, A. L., Calabrese, K. D. S., & Almeida-Souza, F. (2019). Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity. Evidence-Based Complementary and Alternative Medicine, 2019, 1–12. https://doi.org/10.1155/2019/2421695 [Google Scholar]
  29. Wang, C., Su, Y., Shahriar, S. S., Li, Y., & Xie, J. (2024). Emerging strategies for treating medical device and wound‐associated biofilm infections. Microbial Biotechnology, 17(10), e70035. [Google Scholar]
  30. Wylie, M. R., & Merrell, D. S. (2022). The antimicrobial potential of the neem tree Azadirachta indica. Frontiers in Pharmacology, 13, 891535. [Google Scholar]
  31. Yang, X., Wang, Y., Li, J., Zhang, L., & Zhao, Y. (2023). Plant-derived natural products as potential antimicrobial agents against drug-resistant pathogens: A review. Frontiers in Microbiology, 14, 1187853. https://doi.org/10.3389/fmicb.2023.1187853 [Google Scholar]
  32. Zhou, K., Shi, M., Chen, R., Zhang, Y., Sheng, Y., Tong, C., & Shou, D. (2025). Natural phytochemical-based strategies for antibiofilm applications. Chinese Medicine, 20(1), 96. [Google Scholar]

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