Isolation and Screening of Microorganisms from Waste Dumping Sites for Biosorption of Cadmium and Lead

Open Access
Download PDF
AgroEnvironmental Sustainability
Meenu Butola , Neelesh Babu , Vishal Kumar Deshwal

Abstract

Heavy metal poisoning of the environment poses a serious threat to ecosystems and human health. Landfills have been found to harbor diverse microbial communities capable of bioremediating such contaminants via biosorption. Unlike traditional methods of heavy metal remediation, which often involve costly and resource-intensive techniques, biosorption offers a promising alternative that harnesses the natural capabilities of microorganisms. The objective of this research was to identify and screen microorganisms from landfills for their ability to bio-sorb cadmium (Cd) and lead (Pb). The present study deals with the isolation and screening of the microorganisms capable of removing heavy metals from the area near Dehradun Municipal Solid Waste Management Pvt. Ltd. Among the 17 bacterial isolates and 2 fungal isolates, only 7 bacteria and one fungal strain were able to absorb the heavy metal concentration. Among the 7 bacterial strains, the percentage of microbes with the ability to absorb Pb ranged from 35% to 70%, while the percentage of microbes with the ability to absorb Cd ranged from 20% to 50%. On the other hand, the fungal strain had an absorption capacity of about 80% for both Pb and Cd. This study emphasizes the potential of microorganisms isolated from landfills as attractive candidates for Cd and Pb biosorption. These isolates show significant tolerance and efficient biosorption capacities, making them good candidates for further exploration and prospective application in the bioremediation of heavy metal-contaminated environments.

Keywords

biosorption heavy metals isolation screening

References

  1. Alzahrani, M. R., Elsayed, E. A., & Alharbi, N. K. (2021). Biosorption of Pb2+ from aqueous solution using Bacillus subtilis: Optimization and kinetic studies. Journal of Environmental Chemical Engineering, 9(2), 104931. https://doi.org/10.1016/j.jece.2020.104931 [Google Scholar]
  2. Aneja, K. R. (2007). Experiments in Microbiology, Plant Pathology and biotechnology. New Age International, New Delhi, India, pp. 69. [Google Scholar]
  3. Ansari, M. I., & Malik, A. (2007). Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresource Technology, 98(16), 3149-3153. https://doi.org/10.1016/j.biortech.2006.10.008 [Google Scholar]
  4. ATSDR (2020). Toxic substances portal - Heavy metals. Agency for Toxic Substances and Disease Registry. Available at: https://www.atsdr.cdc.gov/toxfaqs/tfacts22.pdf (accessed on 2 May 2023). [Google Scholar]
  5. Brady, D., Stoll, A., & Duncan, J. R. (1994). Biosorptton of heavy metal cations by non‐viable yeast biomass. Environmental Technology, 15(5), 429-438. https://doi.org/10.1080/09593339409385447 [Google Scholar]
  6. Chen, Y., Li, X., Li, R., & Li, X. (2021). Efficient adsorption of Cd (II) from aqueous solutions by Pseudomonas putida: Equilibrium, kinetic and thermodynamic studies. Ecotoxicology and Environmental Safety, 223, 112556. https://doi.org/10.1016/j.ecoenv.2021.112556 [Google Scholar]
  7. Das, N., & Mondal, S. (2018). Biosorption of heavy metals—an overview. Journal of Biotechnology Reports, 19, e00264. https://doi.org/10.1016/j.btre.2018.e00264 [Google Scholar]
  8. El-Sheekh, M. M., Hamouda, R. A., Shaltout, K. H., & Mahmoud, A. M. (2019). Chlamydomonas reinhardtii as an efficient biosorbent for cadmium removal from aqueous solutions. Journal of Applied Phycology, 31(6), 3457-3469. https://doi.org/10.1007/s10811-019-01922-8 [Google Scholar]
  9. Gupta, V. K., Rastogi, A., Nayak, A., & Singh, V. K. (2019). Microbial extracellular polymeric substances (EPSs): A review. Journal of Environmental Management, 220, 35-54. https://doi.org/10.1016/j.jenvman.2018.06.050 [Google Scholar]
  10. Hassen, A., Saidi, N., & Cherif, M. (1998). Biodégradation of petroleum hydrocarbons by Candida tropicalis and Pseudomonas fluorescens isolated from a Tunisian oil refinery. Biotechnology Letters, 20(11), 1113-1116. [Google Scholar]
  11. Järup, L. (2003). Hazards of heavy metal contamination. British Medical Bulletin, 68(1), 167-182. https://doi.org/10.1093/bmb/ldg032 [Google Scholar]
  12. Khan, Z., Elahi, A., Bukhari, D. A., & Rehman, A. (2022). Cadmium sources, toxicity, resistance and removal by microorganisms-A potential strategy for cadmium eradication. Journal of Saudi Chemical Society, 26(6), 101569. https://doi.org/10.1016/j.jscs.2022.101569 [Google Scholar]
  13. Liu, W., Zhang, J., Zhang, Q., & Luo, L. (2018). Characterization of Rhodococcus erythropolis for biosorption of cadmium ions from aqueous solution. Journal of Environmental Chemical Engineering, 6(1), 1141-1148. https://doi.org/10.1016/j.jece.2018.01.050 [Google Scholar]
  14. Rani, M., Kumar, V., & Yadav, A. (2010). Study of heavy metal resistance in fungi isolated from industrial waste. Journal of Applied Sciences and Environmental Management, 14(4), 25-29. [Google Scholar]
  15. Schott, E. J., & Gardner, R. C. (1997). Aluminum-sensitive mutants of Saccharomyces cerevisiae. Molecular and General Genetics, 254, 63-72. https://doi.org/10.1007/s004380050391 [Google Scholar]
  16. Srivastava, S., & Thakur, I. S. (2006). Biosorption potency of Aspergillus niger for removal of chromium (VI). Journal of Colloid and Interface Science, 296(1), 76-84. https://doi.org/10.1016/j.jcis.2005.08.025 [Google Scholar]
  17. Volesky, B. (2007). Biosorption and me. Water Research, 41(18), 4017-4029. https://doi.org/10.1016/j.watres.2007.05.062 [Google Scholar]
  18. Zhou, Q., Yang, X., Li, B., & He, Y. (2020). Highly efficient biosorption of Pb (II) by Aspergillus niger biofilm: Kinetics, isotherms and mechanism. Journal of Environmental Chemical Engineering, 8(5), 104225. https://doi.org/10.1016/j.jece.2020.104225 [Google Scholar]
  19. Zouboulis, A. I., Loukidou, M. X., & Matis, K. A. (2004). Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soils. Process Biochemistry, 39(8), 909-916. [Google Scholar]